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Know About The Use of 13C Metabolite Yeast Extract in Metabolic Profiling

Uniformly (U) 13C-labeled metabolite yeast extract is used as internal standard (IS) in MS (Mass Spectrometry)-based metabolic profiling studies. It helps with successful labeling, quantitation, and identification of biochemical compounds in many cell populations.

In this post, we are going to talk about IROA and IROA quantitation kits supplied with uniformly labeled energy sources for mammalian metabolic profiling. The kits are provided with medium based on Earle’s Balanced Salts (EBSS) and Roswell Park Memorial Institute (RPMI) vitamins as well. These kits, when combined with sample preparation and mass spectrometry, provide all the reagents necessary for quantitating biochemical compounds successfully.

At the end of the post, we will also discuss how you can get the kits. Consider going through the following portion of this article properly to know more.

13C Metabolite Yeast Extract

About IROA

Before we start talking about the quantitation kits, let’s take a look at what IROA is and how it works.

IROA refers to Isotopic Ratio Outlier Analysis. It’s actually a direct and very simple metabolic profiling technique. This technique involves embedding unique signatures, specifically using 95% and 5% 13C abundance, into biochemical metabolites for relative quantitation by MS (Mass Spectrometry) and software algorithms for finding, quantitating, and identifying these signatures or tags. This metabolic profiling technique (IROA) can be applied to a wide range of life science applications.

Why use 95% and 5% 13C labeling?

Here are a few reasons why 95% and 5% 13C abundance is used in IROA:

  • First of all, 95% and 5% 13C helps eliminate false data by generating a unique isotopic pattern which is easily differentiated from natural abundance non-biological artefacts and noise.
  • Second, another great benefit of 95% and 5% 13C labeling is that it removes variances.
  • Third, it enables identification. You will be able to determine the number of carbons for each metabolite.
  • Fourth, it’s cost-effective since you can mix and analyze the samples together.

IROA Quantitation Kits for Mammalian Metabolic Profiling

Here are the IROA quantitation kits you should know about:

IROA® Phenotypic Quantitation Kit

The kit components include:

Labeling Medium –

1. (1) Bottle of 250 mL EBSS/RPMI 1640 vitamin solution (1X) containing 1mg/mL 95% U-13C labeled glucose

2. (1) Empty sterile bottle to store media following filter sterilization

Component Mix –

1. 657.5 mg 95% U-13C labeled yeast extract and amino acids

You will also get ClusterFinder™ software and user manual.

This kit is best for situations where you can’t label experimental samples in culture systems such as biopsies or large-scale fermentation.

IROA® Fluxomic Quantitation Kit

The kit components include:

Labeling Medium –

1. (1) Bottle of 250 mL EBSS/RPMI 1640 vitamin solution (1X) containing 1mg/mL 5% U-13C labeled glucose

2. (1) Empty sterile bottle to store media following filter sterilization

Component Mix –

1. 657.5mg 5% U-13C labeled yeast extract and amino acids

It provides all the reagents to fully label mammalian cells at 5% U-13C.

13C Metabolite Yeast Extract iroatech

Know more about IROA Kits

You will find detailed information about 13C metabolite yeast extract and IROA kits on this website -iroatech.com. ‘IROA Technologies LLC’ is one of the renowned metabolomics company. Feel free to contact them directly for more information.

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